The present study describes rapid screening of Penicillium verruculosum strain BS3 (novel strain previously reported from this laboratory) for the production of secondary metabolites in potatodextrose agar and broth media. Metabolites were extracted from both mycelia (intracellular) and supernatant (extracellular). For intracellular secondary metabolites, the 5 days old mycelia were extracted in the solvent system containing methanol, dichloromethane and ethyl acetate (1:2:3 ratio, respectively) with 1% volume of formic acid; while the extracellular metabolites in the supernatant were extracted in ethyl acetate. The concentrated intraand extra-cellular extracts were pooled in methanol for analysis using liquid chromatograph/quardapole -time of flight/mass spectrometry (LC/Q-TOF/MS). Alkaloids such as aurantioclavine and rugulosuvine B were identified from the extract of P. verruculosum BS3, upon the database search of 465 secondary metabolites produced by various fungi. It indicates that P. verruculosum BS3 is a potent fungus capable of producing valuable leads for drugs such as aurantioclavine, an intermediate for the synthesis of fungal communesin and rugulosuvine B, an antitumor and antimicrobial agent.
Ravunni Maya, Sailas Benjamin