Ethyl ether extraction from seed of Podophyllum hexandrum Royle (P.hexandrum) was separated by GC-MS and nineteen compounds were identified including fatty acid, alkenes, etc. most of which had been proved to have inhibited bioactivities that could be the primary reason of unhealthy germination for P.hexandrum seeds. Hydroquinone was one of the nineteen compounds and the first time identified from P.hexandrum seed in this experiment which had never been reported about its bioactivity before. The further study of its bioactivities was conducted on wheat and vitro culture of embryo of P.hexandrum seed showed that hydroquinone played a positive role and shortened the cycle in growth and development compared to IAA, NAA, BAP, GA3, Which indicates that hydroquinone has similarity to auxin on plant growth and development.
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