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Statistical Approach for Optimization of Independent Variables on Alkali-Thermo Stable Protease Production from Bacillus Licheniformis Strain BIHPUR 0104

An alkalophilic and thermostable protease producing Bacillus licheniformis strain BIHPUR 0104 was isolated from poultry farm. Response surface methodology by Box-Behenken design was employed for the enhanced production of protease using independent variables selected through one variable at one time approach. Under the optimized condition of sucrose (1.5% w/v), temperature (40°C) and incubation period (24 h), the protease production from strain BIHPUR 0104 was enhanced 2 fold compared to one variable at one time approach. The protease experimental yield (483.23 U/ml) closely matched the predicted value (480.74 U/ml) by the statistical model (R2=0.9997). The p values showed that the independent variables had highly significant effect on proteolytic activity (p<0.0001) of the strain tested. The crude protease obtained from strain BIHPUR 0104 showed stability at a wide range of pH (9) and temperature (50°C) indicating thermo-alkali stable nature of the crude enzyme.

Author(s): Ameer Khusro

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