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Assessment of Molecular Variation in Pea Germplasm Using RAPD Markers

Pea (Pisum sativum L.) is an economically important pulse crop that grown for its protein-rich seeds. The Molecular Variation of 20 Pea genotypes supplied from different geographical regions of Iran and ICARDA was evaluated. 20 Random Amplified Polymorphic DNA (RAPD) primers were used in this study. 12 primers could generate 70 repeatable and detectable bands which 88% of them were polymorphic among various genotypes. Polymorphic information content (PIC) values ranged from 0 to 1 with an average of 0.82. The Jacard similarity coefficients among the genotypes ranged from 0.4 (between FLIP 01-32C and FLIP 93-93C) to 0.91 (between FLIP 03-71C and FLIP 03-64C) that indicate a relatively high genetic variation among the pea genotypes evaluated. Both cluster and Principal components analysis (PCA) clearly separated pea genotypes into two groups. The result showed that Iranian genotypes (Azad and Arman) were placed in first cluster in which its other members supplied from ICARDA; therefore, likely they have a common origin. This finding suggested that RAPD markers could be estimated genetic relationships and differences of pea germplasm.


Zahra Alizadeh, Daryoosh Nabati Ahmadi, Mohtasham Mohammadi, Rahmat Allah Karimizadeh, H Rajabi-Memari, Moslem Bahmankar

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