Electronic Journal of Biology

Some Physiological Characteristics of Pepper (Capsicum annuum L.) Produced in vitro

Apical buds were isolated from aseptically grown seedlings of pepper (Capsicum annuum L.), then they were cultivated on MS medium (Murashige and Skoog), with different concentrations and combinations of hormones. After four weeks, pepper shoots were obtained from apical buds in culture tissue in vitro conditions. After eight weeks the plantlets were transferred from sterile conditions in the laboratory to the outside conditions where the regenerate plants became adapted to the environment. Growth regulators affect the translocation of mineral substances in the vegetative organs of plant species. The aim of this research was to determine the content of certain biogenic elements and some photosynthesis pigments in pepper produced in vivo and in vitro conditions. The results showed that the plants obtained of conventional production produced in vivo, which were used as a control, compared with in vitro produced plants were significant differences in only a few of the examined features. Differences occurred primarily due to different hormonal treatment in the stages of preparation of the seedling. In vitro obtained plants have tended to preserve the juvenility characteristics i.e. rejuvenating, which characteristic is proved with the obtained results from examinee parameters

Author(s): Liljana Koleva Gudeva, Snezana Stavreva Veselinovska

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