Microorganisms are omnipresent. They may be useful (e.g. Lactobacilli) or harmful (e.g. Pseudomonas, Salmonella etc) for human beings. The warm and moist environment of our surroundings including floors provides optimal conditions for their growth. There are many microbial species commonly present on the floor surfaces (Serratia marcescens, Staphylococcus aureus etc), most of which are nonpathogenic or pathogenic at higher concentrations. In order to control growth of such organisms floor disinfectants like Lizol, Dettol, Domex are commonly used. According to the Indian Medical Association, Lizol and Dettol are claimed to be the most effective disinfectants. Reports from our laboratory using in vitro analysis to check efficacy of various floor cleansers suggested Dazzl to be equally effective disinfectant as Lizol. In practice, it is impossible to maintain the conditions of the floor as it is maintained in vitro. Therefore, in the present study, experiments were designed using in vivo analysis to confirm the findings. A survey was conducted to determine consumer’s preference and preferred products were further analyzed for their effectiveness in inhibiting the growth of floor microorganisms (in vivo). Attempts were also made to study the longevity of these floor disinfectants using turbidometric analysis. Our results suggested Dazzl to be the more effective as compared to other products used for the investigation.