In present study, 37s fungal strains were isolated on PDA plate containing the 0.02% guaiacol. Out of these 37 isolates, only eight fungal isolates showed reddish brown zones on the medium. The most potent fungal strain for laccase production was screened on 0.06% guaiacol containing PDA medium. On the basis of cultural characteristics, morphological and microscopic sporulating structure it was determined that the isolated fungus belonged to the Apophysomyces sp. This novel laccase producing fungal isolate can be used for large scale production of laccase and in the treatment of various industrial effluents.
Ram Kumar Pundir, Viraj Krishna Mishra, Satish Rana and Megha Lakhani