Liquid chromatograph/quardapole-time of flight/mass spectrometry (LC/Q-TOF/MS) is a modern analytical approach in the rapid screening of crude extracts of biological sample for the characterization of secondary metabolites. LC/Q-TOF/MS offers new possibilities for studying fungal metabolites in its crude form, as it requires no sample cleaning. Furthermore, targeted analysis is also needless as the instrument can provide accurate mass measurement of molecular ions of any components detected in an LC run, and these can be identified by searching a database of exact masses of relevant secondary metabolites, which is linked to the analytical system. In the present study, Purpureocillium lilacinum strain BP13 (a novel isolate from this laboratory) was grown in potato dextrose medium for 14 days and the supernatant was extracted in ethyl acetate and subjected to LC-Q-TOF-MS analysis. The peak at 6.03 min had a mass of m/z 305.2118, exactly corresponding to flavoglaucin with chemical formulae C19H28O3, based on the available fungal database search. Literature shows that flavoglaucin is an excellent antioxidant and a synergizing agent, and also shown to have antibacterial and anticancer properties. This is the first report on the production of flavoglaucin by P. lilacinum.
Thachat Jayakrishnan, Sailas Benjamin