Production of Cellulases and Hemicellulases from Cellulytic Fungal Cultures in Submerged Fermentation Using Agricultural Wastes

Muhammad Irfan; Muhammad Nadeem; Quratualain Syed

Abstract

Production of Cellulases and Hemicellulases from Cellulytic Fungal Cultures in Submerged Fermentation Using Agricultural Wastes

Untreated, Chemically treated and enzyme treated (Saccharified) sugarcane bagasse, wheat straw and rice straw were evaluated in submerged fermentation for the production of cellulases and hemicellulase by monocultures and mixed cultures of Aspergillus niger and Trichoderma viride in submerged fermentation. Maximum CMCase activity of 6.8 ± 0.5 U/mg and xylanase activity of 105± 2.8 U/mg was obtained in enzyme treated sugarcane bagasse and pretreated wheat straw with mixed culture respectively. However, monocultures of Aspergillus niger and Trichoderma viride showed less enzyme production as compared to mixed cultures in rest of the experimentation.

Author(s): Muhammad Irfan, Muhammad Nadeem, Quratualain Syed

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