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Potent in vitro Anti-tumor Activity of Symplocos racemosa against Leukaemia and Cervical Cancer

Objective: Assessment of anti-tumor activity of Symplocos racemosa Roxb. (Symplococaceae) plant extracts. Methods: Plant bark was procured, identified and verified. Chloroform, butanol and ethyl acetate extracts were prepared and their cytotoxic activity determined using the XTT salt based cytotoxicity assay in 96- micro plate format against one leukaemia and one cervical cancer cell line. Cyclophosphamide was used as positive control. Absorbance was measured after 48 h at 450 nm. Results: Total 3 extracts evaluated, butanolic and ethyl acetate extracts demonstrated strong cytotoxic potential overall. Cell proliferation assay showed dose depended inhibition of cell growth. Butanolic extract was found cytotoxic against HL 60(Human leukemia cell line, IC50= 27183 ng/ml), HeLa (Human cervix cancer cell line, IC50= 22861 ng/ml), Ethyl acetate extract was found to be less cytotoxic against HL 60 (IC50= 117084 ng/ml), HeLa (IC50= 137151 ng/ml). Chloroform extract displayed no cytotoxicity against both cell lines. Conclusion: S.racemosa shows potential anti-tumor activity against leukaemia and cervical cancer.

Author(s): Bhuvan P. Raval, Maulik P. Suthar, Rakesh K. Patel

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