Electronic Journal of Biology

Optimization of Parameters for the Production of Cellulase from Achromobacter xylosoxidans BSS4 by Solid-State Fermentation

Now-a-days, an increase in demand for the costeffective methods in the production of industrially useful enzymes like cellulases is witnessed. Thus, the present study was aimed at optimizing the growth parameters like temperature, pH, moisture and duration of incubation for the production of cellulase by Achromobacter xylosoxidans strain BSS4, employing solid-state fermentation (SSF) strategy. Substrates like rice bran, corn bran, sugarcane bagasse, rice straw and saw dust were selected for examining the most efficient one among them in supporting the maximum cellulase production. From the substrates, corn bran supported the maximum cellulase activity (107.7 U/gds) under unoptimized condition; hence, corn bran was selected as substrate for the statistical optimization of different parameters for maximizing the cellulase yield. Box-Behnken design coupled with response surface methodology was applied for the statistical optimization of the process parameters [temperature (40°C), pH (5), moisture (60%), incubation time (6 h)]; which resulted in a 3.7 fold increase in cellulase activity, i.e., from 107.7 U/gds, the activity increased to 512.98 U/gds. Briefly, this study shows that A. xylosoxidans BSS4 is a good producer of cellulase under SSF using corn bran as the substrate, which paves a way for the exploitation of A. xylosoxidans strain BSS4 with an industrial perspective.

Author(s):

Erandapurathukadumana Sreedharan Hareesh, Panichikkal Abdul Faisal, Sailas Benjamin

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