The effect of cytokinins and combination of cytokinins and auxins on in vitro microtuber formation and growth of two potato cultivars of potato (Solanum tuberosum L.) were evaluated. In the present study sprouts and nodal explants of potato, cultivars Agrija and Andrea, were cultured on MS medium, supplemented with different hormonal combinations. For sprouts as an initial explants were used MS + 4 mg/l KIN and MS + 2 mg/l BAP, and for nodal explants were used MS + 4mg/l KIN + 1mg/l IAA and MS + 2 mg/l BAP+1 mg/l NAA. For rapid sprouting clean popato tubers were in vivo treated with 2 ppm GA3. Between the two different explants (nodal segment and sprout) nodal cutting showed the better microtuber formation. The cultivar Agrija showed greater ability for in vitro propagation, with 2.14 tubers per shoot and 13.33% microtuber formation.