The present study describes the culture characteristics, isolation and partial purification of a yellow pigment produced by a novel bacterium, Micrococcus luteus strain BAA2. The growth conditions of M. luteus strain BAA2 were optimized. It showed the optimum growth (OD: 0.55 at λ660) at 28oC (pH 7, 24 h), while the maximum pigment (OD: 0.50 at λ466) production was at 36 h (pH 7). The yellow pigment secreted in the medium was extracted in methanol, and purified using silica column chromatography, and the pigment was characterized using thin layer chromatography (2 spots: Rf 0.38 and 0.43), UV-visible and IR spectroscopic techniques; and both spectroscopic profiles showed the characteristic peaks of carotenoid pigment.
Pawar Y Surekha, Dhanya P, Sarath Josh MK, Pradeep S, Sailas Benjamin