The paper systematically studies the manufacture of aqueous vinyl polymer solution-isocyanate adhesive (API) through utilizing esterified corn starch. Taking the certain acid and basicity as condition, corn starch was esterified to form emulsion that was condensed with synthesized no poison, no environmental pollution rubber emulsion to manufacture the main agent of API; Isocyanao compound which isocyanate group were taken as curing agent of API adhesive. Main agent and curing agent made up double component API adhesive which have no formaldehyde emission and improve water resistance. After production experiments in three layers composite flooring board, blockboard, plywood, laminated stuff and other bond products with this API adhesive proved, all performance indexes were passed and there were no poison materials emission from products. This API adhesive not only operated easily in production but also met the need of present technology. Besides, its cost is half of other import adhesives, so API adhesive we had manufactured obviously social environmental benefits and economic profits.