The enzyme activities of two hydrolases and phenylalanine ammonia-lyase were analyzed in the seedlings of four mungbean cultivars (TARM-1, Kopergaon, ML-936 and Pant M-3) differing in their resistant status. Nine day after germination, the seedlings were challenged with Cercospora canescens cell wall elicitor and spore suspension and they were evaluated periodically for the enzyme activities. The hydrolases i.e. chitinases and -1,3 glucanases were found to be induced in all seedling parts by both inducers. The activities of these enzymes were higher in resistant cultivars. The maximum activity of glucanase, chitinase and PAL was observed in cotyledons of ML-936 followed by Pant M-3. This induced activity of defense related enzymes was found to be associated with resistant status of mungbean cultivars.