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In vitro Variation in antibacterial activity plant extracts on Glaucium elegans and Saffron (Crocus sativus .L) Onios

The increase in antibiotic resistance has resulted in a decreasing number of fully active antimicrobial agents available to treat infections by multi-drug resistant (MDR) bacteria. This has necessitated a search for new antimicrobial agents. Though these plants are used for various purposes, but there are plants that the medicinal properties of the plants were not investigated. The major aim of this study was to determine the antimicrobial activity of the extracts of Glaucium elegans and Saffron (Crocus sativus .L) Onios plant species against Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Bacillus anthracis and Proteus. The extracts of medicinal plants were prepared using Soxhlet apparatus for methanol extract. The antibacterial activities by agar disc diffusion and serial dilution assays; the inhibitory zones were recorded in millimeters. The methanol extract of Glaucium elegans was found to have a significant antibacterial efficiency (p ≤ 0.05) as compared to the methanol extract of Onios plant. The methanol extract of Glaucium elegans showed high antibacterial potential (23 mm) against Proteus at concentration of 1500μg/disc. On the other hand showed highest antibacterial activities (22 mm) against Staphylococcus aureus at concentration of 1500μg/disc. These finding pinpoint the efficiency of these extracts to inhibit microbial growth. The bactericidal activity described here represents an added safety value for Glaucium elegans as a means of eliminating a possible antimicrobial during storage.

Author(s): Ehsan Heidari Soureshjan, Mina Heidari

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