The effect of Humates and silver nitrate on organogenesis of cotyledon and hypocotyl explants of four sweet pepper (Capsicum annuum L.) varieties was investigated. The rate of plant regeneration was found to depend on genotype, explant type and culture medium. The cotyledons were more effective as explants for bud induction and subsequent plant elongation than hypocotyls. The highest regeneration frequency (2.35 plants per explant) was achieved in cotyledons from variety Maritsa on culture medium supplemented with 0.2 mgL-1 AgNO3. The medium containing 5.0 mlL-1 Humates promoted plant regeneration in hypocotyls. Differences in morphological characteristics and pollen fertility between seed-derived and in vitro regenerated plants grown in greenhouse conditions were registered.