Microorganisms, the friend and foe of human being are omnipresent in and around us. Innumerable pathogenic and non pathogenic microorganisms remain present on the floor surface with which we are in direct physical contact. These organisms include Salmonella, Rhinovirus, Herpes, Trichophyton, Giardia, E. coli, Micrococcus species etc. Most of the microbes are non pathogenic when present in less quantity, but as their concentration increases they are able to cause various diseases like Stomach upsets, diarrhoea, Cold, Cold sores etc. To restrict the growth of such microbes in the surroundings including floors, various commercially available floor cleaners such as Phenyls, Dettol, Kli-Nol, Dazzl, etc. are used. Present study is an attempt to isolate and identify the floor micro flora from home and laboratory and to observe the efficacy of different floor cleaners. According to Indian Medical Association (IMA) “Dettol and Lizol” are identified as the most effective disinfectant against the floor micro flora, and therefore they are considered as standards to analyze effectiveness of other commercial surface cleaners. Our study suggested that Dazzl can inhibit the growth of common floor micro flora as effectively as Dettol and better than Lizol.