Frequency of mutation in alpha-globins gene has a great diversity in different populations, and unlike other hemoglobinopathies is not recognizable by simple biochemical experiments. Carriers of these genes have anemia microcytic and its clinical syndromes are variable from being without any sign to fatal hemolytic anemia. Diagnosis of these carriers is useful in order to find out what causes microcytic, avoid long-term treatment of iron deficiency anemia, and prevent marriage between transporters of alpha thalassemia and birth of infants suffering from severe anemia.
In this study, 136 blood samples of people with reduced blood indexes HBA2 Á MCH<27pg Á MCV<80fl were gathered and studied randomly from laboratories in Tonekabon, Ramsar, and Chalous. Identification of deletion mutations is done by sage of multiplex- PCR method. Deletion mutations include single gene deletions -α3.7 and -α4.2, homozygous double gene deletions -α3.7/-α3.7 and - -THAI in 56 samples of all studied samples. Also, a triple gene deletion -α3.7/- - FIL, which has never been recognized, is identified in this study. The most prevalent deletion in Tonekabon is single gene deletion -α3.7. This fast, low-cost, and simple method is useful for status determination of those who are indistinctively classified in globin alpha gene.
Zahra Nashtahosseini, Ali Nazemi, Shahrbanoo Keihanian, Reza Hajihosseini, Mohammad Eskandari