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Evaluation of Genetic Variation in Rye Germplasm using ISSR Markers

Cultivated rye (Secale cereal L.) is a diploid plant used for feeding humans and animals. Closely studying genetic variation is the main component of breeding programs and desirable genetic variation is a prerequisite for selection. In this study, Molecular Variation of 22 Iranian and exotic rye genotypes were evaluated using 15 ISSR primers which 13 of them produced suitable repeatable bands. Result indicated that 761 bands (70%) out of total 1091 bands were polymorphic. Polymorphic information content (PIC) and Shannon's information index (I) were ranged from PIC=0.45, I=0.64, PIC=0.29, I=0.43, respectively. Based on Jaccard similarity matrix, the highest similarity (0.74) was observed among Ethiopia 3 and Palestine genotypes and the lowest similarity was observed among Shapour and Ardebil genotypes with 0.24. Performing cluster analysis using Jaccard similarity matrix and Complete Linkage method resulted in the most suitable groupings which the genotypes were divided in five groups. Generally the results of the study indicated that ISSR markers can be used for grouping rye genotypes.


Nastaran Partovi, Mohsen Ebrahimi, Zohreh Alemi

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