The growth performance of O. niloticus fingerlings fed with diets having fermented fish offal was investigated for a 90 days. One reference (RFM) diet (Mustard oil cake + wheat flour + rice bran + egg shell dust) and one experimental diet containing fermented fish offal (39%) were (FOM)formulated. The diets were fed twice (06.00 and 17.00 hours) daily ad libitum in two replicates. The growth experiment with these diets showed that the diet with fermented fish offal rendered highest growth of the tilapia. The diet, containing fermented fish offal showed significantly higher (P<0.05) growth, feed conversion ratio (FCR), protein efficiency ratio (PER), specific growth rate (SGR), hepatosomatic index (HSI) and gonadosomatic index (GSI) than the reference diet without fermented fish offal. Fatty acid profile of FOM fed fish was also better as compared to the reference diet. It was concluded that effectiveness of fermented fish offal could be substantially increased the growth and fish quality by accumulating more poly unsaturated fatty acid (PUFA) in fish flesh of tilapia (O. niloticus).