Electronic Journal of Biology

Differential Gene Expression Patterns in the Basal Stems of Wheat at the Jointing Stage

Improved differential display of mRNA was used to analyze alterations in gene expression during the internode elongation of basal stem of wheat plant. Three patterns of differential expression were observed: (I) bands observed in pre-elongation basal stem tissue, but not in elongated; (II) bands occurring in elongated but not in pre-elongation basal stem tissue; (III) bands vibrated during the phase. Three differentially expressed cDNA fragments (WSR1-3) had been cloned and sequenced. Sequence homology search in GenBank database showed that one (WSR2) of them has no homology hits; one transcripts (WSR1) had similarity to barley serine/threonine protein kinase; while the product of WSR3 showed high similarity to Ty3/gypsy-type retrotransposon reverse transcriptase, which indicated diverse pathways may be involved in the internode elongation of wheat stem.

Author(s): Yongchun Li, Fanrong Meng, Jiangping Ren, Jun Yin, Hongbin Niu

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