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Cytoplasm Transfer in Mature Rabbit Oocytes

oocyte fertilization and early embryonic development and study the mitochondrial DNA heterogeneity of early embryos, microinjection was performed to transfer a small amount (5% to 7%) of donor ooplasm into recipient oocytes and fertilize the eggs with rabbit sperm through intracytoplasmic sperm injection (ICSI). In Group 1 (homogeneous ooplasmic transfer), both the donor and recipient rabbit oocytes were at metaphase II. In Group 2 (heterogeneous ooplasmic transfer), the donor was mouse oocyte of metaphase II and the recipient was rabbit oocyte of metaphase II. In the control group, only ICSI was done on rabbit oocyte without ooplasmic transfer. Result showed that homogenous ooplasmic transfer had no significant influence on rabbit oocyte fertilization and early embryonic development, but heterogeneous ooplasmic transfer did cause notable reduction in blastocyst development rate. Compared with the control group, the embryonic quality declined after ooplasmic transfer operation in the present experiment.

Author(s): Junfeng Li, Yanhua Song, Haifeng Li, Jiahua Zhang

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