Satureja bachtiarica Bunge (local name: Marzeh-e-Koohi) is an endemic specie plant that widely distributed in the Central Zagros Mountains, Iran. This study was designed to examine the chemical composition and antibacterial activity of the essential oil of Satureja bachtiarica Bunge (Lamiacae). The essential oil of Bakhtiari savoury was analyzed by GC/MS, and also tested for antibacterial activity of the essential oil was tested by agar disc diffusion and serial dilations assay against four positive-Gram (Bacillus cereus, Staphylococcus aureus, Streptococcus agalactiae and Listeria monocytogenes) and two negative-Gram (Proteus vulgaris and Salmonella typhimurium) bacteria strains. The results showed that the major components of the oil were carvacrol (44.8%), ?-terpinen (18.7%) and thymol (14.95%). The essential oil of S. bachtiarica has strong antibacterial against bacteria strains, especially S. agalactiae, S. typhimurium and P. vulgaris. MICs of the essential oil against B. cereus = 31.25 μg/ml, S. aureus = 250 μg/ml, S. agalactiae = 31.25 μg/ml, L. monocytogenes = 62.5 μg/ml, P. vulgaris = 62.5 μg/ml and S. typhimurium = 16 μg/ml were determined. Finally, the essential oil of S. bachtiarica aerial parts could be suitable for application in the food industry and pharmaceutical.