The aim of our researches was to follow the establishment of the symbiotic associations in “in vitro” dual cultures. The partners consisted in root cultures with continuous growth and calli derived from the root tissues in Ipomoea batatas, Zea mays, and Cucumis sativus and spores of the VAM fungus Glomus intraradices. Our results highlighted that following the processes of dedifferentiation in other cell types, specific to cells and tissue culturing in artificial conditions, the plant cells preserve not only their intrinsic capacity of morphogenetic capacity, but also the capacity of biochemical totipotency. This is expressed by signal exchanges and recognition between the plant cells and the VAM fungus, thus permitting the establishment of the symbiosis.
Ana Rosu, Aurelia Brezeanu, Cristian Banciu, Raluca Stoiculescu, Silvana Danaila-Guidea