Amygdalus communis, A. haussknechtii, A. arabica, A. orientalis, and A. lycioides belong the family Rosaceae, are Iranian native plants. In this study, antioxidant activity of hydroalcholic extracts from the leaves of A. haussknechtii, A. arabica, A. orientalis and A. lycioides, and five cultivars of A. communis was evaluated by DPPH assay. Results indicated the hydroalcholic extract from the leaves of A. haussknechtii, and A. lycioides had the highest antioxidant activity. The total phenolic content of the extracts ranged from 19 to 30 mg Gallic acid/g dry weight extract. The hydro-alcoholic extract of A. haussknechtii exhibited the highest total phenolic content. These results suggested that the level of antioxidant activity in these plants was varied to a great extent. Our results indicated the extract of A. haussknechtii could be an important dietary source of total phenolic content with high antioxidant capacity.