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Abstract

Relationship of ApaI VDR Gene Polymorphism to Type 2 Diabetes Mellitus in Pakistani Population

The following study is aimed to describe the relationship of vitamin D receptor (VDR) genetic polymorphism of ApaI to the onset of type 2 diabetes mellitus and obese patients in the local population. One hundred and fifty Type 2 diabetes mellitus (T2DM) subjects and one hundred controls were taken in this study. Genomic deoxyribonucleic acid (DNA) was recovered by standard salt and ethanol precipitation. VDR polymorphism ApaI (rs7975232 G>T) was studied by restriction fragment length polymorphism (RFLP). The presence of all three fragments (217, 528 and 745 bp) after digestion with restriction enzyme indicates heterozygosity (Aa) of ApaI genotype. Results revealed no significant association of ApaI with the T2DM onset and metabolic parameters to support the hypothesis that ApaI may prone macrovascular diabetic complications.


Author(s):

Madiha Sikander



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